standard least squares regression model using jmp pro 16.0.0 Search Results


standard edition se version v16  (STATA Corporation)
99
STATA Corporation standard edition se version v16
Standard Edition Se Version V16, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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standard edition se version v16 - by Bioz Stars, 2026-08
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mouse anti mouse galectin 3 antibody mac 2  (Proteintech)
94
Proteintech mouse anti mouse galectin 3 antibody mac 2
Pyroptosis is activated in fat grafts. (A) Schematic of an animal model for fat grafting. (B) Scanning electron microscopy (SEM) image of the grafts 1 week after transplantation. Yellow arrows denote bubble-like protrusions, white arrows are pores in the cell membrane. Scale bar: 2 μm. (C, D) The morphology of macrophages in fat-grafted tissues was observed under transmission electron microscope (TEM). Arrows indicate the broken cell membrane. (E-H) Representative immunofluorescence staining of NLRP3 (red) <t>and</t> <t>MAC-2</t> (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (I-L) Representative immunofluorescence staining of cleaved-caspase-1 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (M-P) Representative immunofluorescence staining of IL-18 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (Q, R) WB analysis and quantification of GSDMD activation in fat-grafted tissues at different time points. GAPDH was utilized as a loading control. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01. Casp-1, Caspase-1; DAPI, 4′,6-diamidino-2-phenylindole; GSDMD, gasdermin D; IL-18, Interleukin 18; L, lipid droplet; M, mitochondria; MAC-2, mouse <t>anti-mouse</t> <t>Galectin-3</t> antibody; N, nucleus; NLRP3, NOD-like receptor family, pyrin domain–containing 3; SD, standard deviation; WB, Western blot.
Mouse Anti Mouse Galectin 3 Antibody Mac 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
mouse anti mouse galectin 3 antibody mac 2 - by Bioz Stars, 2026-08
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scanning calorimetry  (Linseis Messgerate)
95
Linseis Messgerate scanning calorimetry
Pyroptosis is activated in fat grafts. (A) Schematic of an animal model for fat grafting. (B) Scanning electron microscopy (SEM) image of the grafts 1 week after transplantation. Yellow arrows denote bubble-like protrusions, white arrows are pores in the cell membrane. Scale bar: 2 μm. (C, D) The morphology of macrophages in fat-grafted tissues was observed under transmission electron microscope (TEM). Arrows indicate the broken cell membrane. (E-H) Representative immunofluorescence staining of NLRP3 (red) <t>and</t> <t>MAC-2</t> (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (I-L) Representative immunofluorescence staining of cleaved-caspase-1 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (M-P) Representative immunofluorescence staining of IL-18 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (Q, R) WB analysis and quantification of GSDMD activation in fat-grafted tissues at different time points. GAPDH was utilized as a loading control. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01. Casp-1, Caspase-1; DAPI, 4′,6-diamidino-2-phenylindole; GSDMD, gasdermin D; IL-18, Interleukin 18; L, lipid droplet; M, mitochondria; MAC-2, mouse <t>anti-mouse</t> <t>Galectin-3</t> antibody; N, nucleus; NLRP3, NOD-like receptor family, pyrin domain–containing 3; SD, standard deviation; WB, Western blot.
Scanning Calorimetry, supplied by Linseis Messgerate, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scanning calorimetry - by Bioz Stars, 2026-08
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lpc 16 0 0 0  (Larodan)
94
Larodan lpc 16 0 0 0
Pyroptosis is activated in fat grafts. (A) Schematic of an animal model for fat grafting. (B) Scanning electron microscopy (SEM) image of the grafts 1 week after transplantation. Yellow arrows denote bubble-like protrusions, white arrows are pores in the cell membrane. Scale bar: 2 μm. (C, D) The morphology of macrophages in fat-grafted tissues was observed under transmission electron microscope (TEM). Arrows indicate the broken cell membrane. (E-H) Representative immunofluorescence staining of NLRP3 (red) <t>and</t> <t>MAC-2</t> (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (I-L) Representative immunofluorescence staining of cleaved-caspase-1 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (M-P) Representative immunofluorescence staining of IL-18 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (Q, R) WB analysis and quantification of GSDMD activation in fat-grafted tissues at different time points. GAPDH was utilized as a loading control. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01. Casp-1, Caspase-1; DAPI, 4′,6-diamidino-2-phenylindole; GSDMD, gasdermin D; IL-18, Interleukin 18; L, lipid droplet; M, mitochondria; MAC-2, mouse <t>anti-mouse</t> <t>Galectin-3</t> antibody; N, nucleus; NLRP3, NOD-like receptor family, pyrin domain–containing 3; SD, standard deviation; WB, Western blot.
Lpc 16 0 0 0, supplied by Larodan, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lpc 16 0 0 0 - by Bioz Stars, 2026-08
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li 1600 porometer  (LI-COR)
94
LI-COR li 1600 porometer
Pyroptosis is activated in fat grafts. (A) Schematic of an animal model for fat grafting. (B) Scanning electron microscopy (SEM) image of the grafts 1 week after transplantation. Yellow arrows denote bubble-like protrusions, white arrows are pores in the cell membrane. Scale bar: 2 μm. (C, D) The morphology of macrophages in fat-grafted tissues was observed under transmission electron microscope (TEM). Arrows indicate the broken cell membrane. (E-H) Representative immunofluorescence staining of NLRP3 (red) <t>and</t> <t>MAC-2</t> (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (I-L) Representative immunofluorescence staining of cleaved-caspase-1 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (M-P) Representative immunofluorescence staining of IL-18 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (Q, R) WB analysis and quantification of GSDMD activation in fat-grafted tissues at different time points. GAPDH was utilized as a loading control. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01. Casp-1, Caspase-1; DAPI, 4′,6-diamidino-2-phenylindole; GSDMD, gasdermin D; IL-18, Interleukin 18; L, lipid droplet; M, mitochondria; MAC-2, mouse <t>anti-mouse</t> <t>Galectin-3</t> antibody; N, nucleus; NLRP3, NOD-like receptor family, pyrin domain–containing 3; SD, standard deviation; WB, Western blot.
Li 1600 Porometer, supplied by LI-COR, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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electromagnetics version 16.0.0  (ANSYS inc)
90
ANSYS inc electromagnetics version 16.0.0
Pyroptosis is activated in fat grafts. (A) Schematic of an animal model for fat grafting. (B) Scanning electron microscopy (SEM) image of the grafts 1 week after transplantation. Yellow arrows denote bubble-like protrusions, white arrows are pores in the cell membrane. Scale bar: 2 μm. (C, D) The morphology of macrophages in fat-grafted tissues was observed under transmission electron microscope (TEM). Arrows indicate the broken cell membrane. (E-H) Representative immunofluorescence staining of NLRP3 (red) <t>and</t> <t>MAC-2</t> (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (I-L) Representative immunofluorescence staining of cleaved-caspase-1 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (M-P) Representative immunofluorescence staining of IL-18 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (Q, R) WB analysis and quantification of GSDMD activation in fat-grafted tissues at different time points. GAPDH was utilized as a loading control. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01. Casp-1, Caspase-1; DAPI, 4′,6-diamidino-2-phenylindole; GSDMD, gasdermin D; IL-18, Interleukin 18; L, lipid droplet; M, mitochondria; MAC-2, mouse <t>anti-mouse</t> <t>Galectin-3</t> antibody; N, nucleus; NLRP3, NOD-like receptor family, pyrin domain–containing 3; SD, standard deviation; WB, Western blot.
Electromagnetics Version 16.0.0, supplied by ANSYS inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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electromagnetics version 16.0.0 - by Bioz Stars, 2026-08
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genome evolution in triticeae  (Schmid GmbH)
90
Schmid GmbH genome evolution in triticeae
Pyroptosis is activated in fat grafts. (A) Schematic of an animal model for fat grafting. (B) Scanning electron microscopy (SEM) image of the grafts 1 week after transplantation. Yellow arrows denote bubble-like protrusions, white arrows are pores in the cell membrane. Scale bar: 2 μm. (C, D) The morphology of macrophages in fat-grafted tissues was observed under transmission electron microscope (TEM). Arrows indicate the broken cell membrane. (E-H) Representative immunofluorescence staining of NLRP3 (red) <t>and</t> <t>MAC-2</t> (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (I-L) Representative immunofluorescence staining of cleaved-caspase-1 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (M-P) Representative immunofluorescence staining of IL-18 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (Q, R) WB analysis and quantification of GSDMD activation in fat-grafted tissues at different time points. GAPDH was utilized as a loading control. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01. Casp-1, Caspase-1; DAPI, 4′,6-diamidino-2-phenylindole; GSDMD, gasdermin D; IL-18, Interleukin 18; L, lipid droplet; M, mitochondria; MAC-2, mouse <t>anti-mouse</t> <t>Galectin-3</t> antibody; N, nucleus; NLRP3, NOD-like receptor family, pyrin domain–containing 3; SD, standard deviation; WB, Western blot.
Genome Evolution In Triticeae, supplied by Schmid GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genome evolution in triticeae - by Bioz Stars, 2026-08
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stare v. 16.00  (METTLER TOLEDO)
90
METTLER TOLEDO stare v. 16.00
Pyroptosis is activated in fat grafts. (A) Schematic of an animal model for fat grafting. (B) Scanning electron microscopy (SEM) image of the grafts 1 week after transplantation. Yellow arrows denote bubble-like protrusions, white arrows are pores in the cell membrane. Scale bar: 2 μm. (C, D) The morphology of macrophages in fat-grafted tissues was observed under transmission electron microscope (TEM). Arrows indicate the broken cell membrane. (E-H) Representative immunofluorescence staining of NLRP3 (red) <t>and</t> <t>MAC-2</t> (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (I-L) Representative immunofluorescence staining of cleaved-caspase-1 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (M-P) Representative immunofluorescence staining of IL-18 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (Q, R) WB analysis and quantification of GSDMD activation in fat-grafted tissues at different time points. GAPDH was utilized as a loading control. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01. Casp-1, Caspase-1; DAPI, 4′,6-diamidino-2-phenylindole; GSDMD, gasdermin D; IL-18, Interleukin 18; L, lipid droplet; M, mitochondria; MAC-2, mouse <t>anti-mouse</t> <t>Galectin-3</t> antibody; N, nucleus; NLRP3, NOD-like receptor family, pyrin domain–containing 3; SD, standard deviation; WB, Western blot.
Stare V. 16.00, supplied by METTLER TOLEDO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eclipse tps version 16 00 00  (Varian Medical)
86
Varian Medical eclipse tps version 16 00 00
Pyroptosis is activated in fat grafts. (A) Schematic of an animal model for fat grafting. (B) Scanning electron microscopy (SEM) image of the grafts 1 week after transplantation. Yellow arrows denote bubble-like protrusions, white arrows are pores in the cell membrane. Scale bar: 2 μm. (C, D) The morphology of macrophages in fat-grafted tissues was observed under transmission electron microscope (TEM). Arrows indicate the broken cell membrane. (E-H) Representative immunofluorescence staining of NLRP3 (red) <t>and</t> <t>MAC-2</t> (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (I-L) Representative immunofluorescence staining of cleaved-caspase-1 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (M-P) Representative immunofluorescence staining of IL-18 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (Q, R) WB analysis and quantification of GSDMD activation in fat-grafted tissues at different time points. GAPDH was utilized as a loading control. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01. Casp-1, Caspase-1; DAPI, 4′,6-diamidino-2-phenylindole; GSDMD, gasdermin D; IL-18, Interleukin 18; L, lipid droplet; M, mitochondria; MAC-2, mouse <t>anti-mouse</t> <t>Galectin-3</t> antibody; N, nucleus; NLRP3, NOD-like receptor family, pyrin domain–containing 3; SD, standard deviation; WB, Western blot.
Eclipse Tps Version 16 00 00, supplied by Varian Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eclipse tps version 16 00 00 - by Bioz Stars, 2026-08
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stare evaluation version 16.00  (METTLER TOLEDO)
90
METTLER TOLEDO stare evaluation version 16.00
Pyroptosis is activated in fat grafts. (A) Schematic of an animal model for fat grafting. (B) Scanning electron microscopy (SEM) image of the grafts 1 week after transplantation. Yellow arrows denote bubble-like protrusions, white arrows are pores in the cell membrane. Scale bar: 2 μm. (C, D) The morphology of macrophages in fat-grafted tissues was observed under transmission electron microscope (TEM). Arrows indicate the broken cell membrane. (E-H) Representative immunofluorescence staining of NLRP3 (red) <t>and</t> <t>MAC-2</t> (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (I-L) Representative immunofluorescence staining of cleaved-caspase-1 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (M-P) Representative immunofluorescence staining of IL-18 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (Q, R) WB analysis and quantification of GSDMD activation in fat-grafted tissues at different time points. GAPDH was utilized as a loading control. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01. Casp-1, Caspase-1; DAPI, 4′,6-diamidino-2-phenylindole; GSDMD, gasdermin D; IL-18, Interleukin 18; L, lipid droplet; M, mitochondria; MAC-2, mouse <t>anti-mouse</t> <t>Galectin-3</t> antibody; N, nucleus; NLRP3, NOD-like receptor family, pyrin domain–containing 3; SD, standard deviation; WB, Western blot.
Stare Evaluation Version 16.00, supplied by METTLER TOLEDO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stare evaluation version 16.00 - by Bioz Stars, 2026-08
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integration system software version 2.16.0.0  (Visiopharm AS)
90
Visiopharm AS integration system software version 2.16.0.0
Pyroptosis is activated in fat grafts. (A) Schematic of an animal model for fat grafting. (B) Scanning electron microscopy (SEM) image of the grafts 1 week after transplantation. Yellow arrows denote bubble-like protrusions, white arrows are pores in the cell membrane. Scale bar: 2 μm. (C, D) The morphology of macrophages in fat-grafted tissues was observed under transmission electron microscope (TEM). Arrows indicate the broken cell membrane. (E-H) Representative immunofluorescence staining of NLRP3 (red) <t>and</t> <t>MAC-2</t> (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (I-L) Representative immunofluorescence staining of cleaved-caspase-1 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (M-P) Representative immunofluorescence staining of IL-18 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (Q, R) WB analysis and quantification of GSDMD activation in fat-grafted tissues at different time points. GAPDH was utilized as a loading control. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01. Casp-1, Caspase-1; DAPI, 4′,6-diamidino-2-phenylindole; GSDMD, gasdermin D; IL-18, Interleukin 18; L, lipid droplet; M, mitochondria; MAC-2, mouse <t>anti-mouse</t> <t>Galectin-3</t> antibody; N, nucleus; NLRP3, NOD-like receptor family, pyrin domain–containing 3; SD, standard deviation; WB, Western blot.
Integration System Software Version 2.16.0.0, supplied by Visiopharm AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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integration system software version 2.16.0.0 - by Bioz Stars, 2026-08
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ansys fluent software  (ANSYS inc)
90
ANSYS inc ansys fluent software
Pyroptosis is activated in fat grafts. (A) Schematic of an animal model for fat grafting. (B) Scanning electron microscopy (SEM) image of the grafts 1 week after transplantation. Yellow arrows denote bubble-like protrusions, white arrows are pores in the cell membrane. Scale bar: 2 μm. (C, D) The morphology of macrophages in fat-grafted tissues was observed under transmission electron microscope (TEM). Arrows indicate the broken cell membrane. (E-H) Representative immunofluorescence staining of NLRP3 (red) <t>and</t> <t>MAC-2</t> (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (I-L) Representative immunofluorescence staining of cleaved-caspase-1 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (M-P) Representative immunofluorescence staining of IL-18 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (Q, R) WB analysis and quantification of GSDMD activation in fat-grafted tissues at different time points. GAPDH was utilized as a loading control. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01. Casp-1, Caspase-1; DAPI, 4′,6-diamidino-2-phenylindole; GSDMD, gasdermin D; IL-18, Interleukin 18; L, lipid droplet; M, mitochondria; MAC-2, mouse <t>anti-mouse</t> <t>Galectin-3</t> antibody; N, nucleus; NLRP3, NOD-like receptor family, pyrin domain–containing 3; SD, standard deviation; WB, Western blot.
Ansys Fluent Software, supplied by ANSYS inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Pyroptosis is activated in fat grafts. (A) Schematic of an animal model for fat grafting. (B) Scanning electron microscopy (SEM) image of the grafts 1 week after transplantation. Yellow arrows denote bubble-like protrusions, white arrows are pores in the cell membrane. Scale bar: 2 μm. (C, D) The morphology of macrophages in fat-grafted tissues was observed under transmission electron microscope (TEM). Arrows indicate the broken cell membrane. (E-H) Representative immunofluorescence staining of NLRP3 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (I-L) Representative immunofluorescence staining of cleaved-caspase-1 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (M-P) Representative immunofluorescence staining of IL-18 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (Q, R) WB analysis and quantification of GSDMD activation in fat-grafted tissues at different time points. GAPDH was utilized as a loading control. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01. Casp-1, Caspase-1; DAPI, 4′,6-diamidino-2-phenylindole; GSDMD, gasdermin D; IL-18, Interleukin 18; L, lipid droplet; M, mitochondria; MAC-2, mouse anti-mouse Galectin-3 antibody; N, nucleus; NLRP3, NOD-like receptor family, pyrin domain–containing 3; SD, standard deviation; WB, Western blot.

Journal: Aesthetic Surgery Journal

Article Title: Disulfiram Improves Fat Graft Retention by Modulating Macrophage Polarization With Inhibition of NLRP3 Inflammasome-Mediated Pyroptosis

doi: 10.1093/asj/sjae075

Figure Lengend Snippet: Pyroptosis is activated in fat grafts. (A) Schematic of an animal model for fat grafting. (B) Scanning electron microscopy (SEM) image of the grafts 1 week after transplantation. Yellow arrows denote bubble-like protrusions, white arrows are pores in the cell membrane. Scale bar: 2 μm. (C, D) The morphology of macrophages in fat-grafted tissues was observed under transmission electron microscope (TEM). Arrows indicate the broken cell membrane. (E-H) Representative immunofluorescence staining of NLRP3 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (I-L) Representative immunofluorescence staining of cleaved-caspase-1 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (M-P) Representative immunofluorescence staining of IL-18 (red) and MAC-2 (green) in fat grafts at 1 week after fat transplantation. Scale bar: 50 μm. (Q, R) WB analysis and quantification of GSDMD activation in fat-grafted tissues at different time points. GAPDH was utilized as a loading control. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01. Casp-1, Caspase-1; DAPI, 4′,6-diamidino-2-phenylindole; GSDMD, gasdermin D; IL-18, Interleukin 18; L, lipid droplet; M, mitochondria; MAC-2, mouse anti-mouse Galectin-3 antibody; N, nucleus; NLRP3, NOD-like receptor family, pyrin domain–containing 3; SD, standard deviation; WB, Western blot.

Article Snippet: In brief, sections were incubated at 4°C overnight with mouse anti-mouse Galectin-3 antibody MAC-2 (cat. 60207-1-Ig, Proteintech, Wuhan, China, 1:1600); rabbit anti-mouse CD206 (cat. DF4149, Affinity, Jiangsu, China, 1:200); rabbit anti-mouse NLRP3 (cat. DF7438, Affinity, 1:100); rabbit anti-mouse IL-18 (cat. DF6252, Affinity, 1:400); and rabbit anti-mouse cleaved-caspase-1 (cat. AF4022, Affinity, 1:200) primary antibodies, and then with Alexa Fluor 448-conjugated goat anti-mouse (cat. A23210, Abbkine, Wuhan, China, 1:200) and Alexa Fluor 594-conjugated goat anti-rabbit (cat. A23420, Abbkine, Wuhan, China, 1:200) secondary antibodies.

Techniques: Animal Model, Electron Microscopy, Transplantation Assay, Membrane, Transmission Assay, Microscopy, Immunofluorescence, Staining, Activation Assay, Standard Deviation, Western Blot

DSF attenuates pyroptosis after fat grafting. (A-G) Double immunofluorescence staining images of fat grafts at 1, 2, and 4 weeks after fat transplantation showed colocalization of NLRP3 and MAC-2 in the indicated groups. Arrows denote NLRP3-positive macrophages. Scale bar: 50 μm. Graph shows the ratio of NLRP3-positive macrophage in the indicated groups. (H, I) WB analysis and quantification of NLRP3, GSDMD, IL-18, IL-1β, and caspase-1 protein levels were conducted in each group of fat grafts after 2 weeks of fat grafting. GAPDH was utilized as a loading control. (J-P) Double immunofluorescence staining images of fat grafts at 1, 2, and 4 weeks after fat transplantation showed colocalization of cleaved-caspase-1 and MAC-2 in the indicated groups. Arrows denote cleaved-caspase-1-positive macrophages. Scale bar: 50 μm. Graph shows the ratio of cleaved-caspase-1-positive macrophage in the indicated groups. (Q-W) Double immunofluorescence staining images of fat grafts at 1, 2, and 4 weeks after fat transplantation showed colocalization of IL-18 and MAC-2 in the indicated groups. Arrows denoted IL-18–positive macrophages. Scale bar: 50 μm. Graph shows the ratio of IL-18–positive macrophage in the indicated groups. The data are expressed as the mean ± SD ( n = 3). * P < .05, ** P < .01, *** P < .001. DSF, disulfiram; GSDMD, gasdermin D; IL, interleukin; SD, standard deviation; WB, Western blot.

Journal: Aesthetic Surgery Journal

Article Title: Disulfiram Improves Fat Graft Retention by Modulating Macrophage Polarization With Inhibition of NLRP3 Inflammasome-Mediated Pyroptosis

doi: 10.1093/asj/sjae075

Figure Lengend Snippet: DSF attenuates pyroptosis after fat grafting. (A-G) Double immunofluorescence staining images of fat grafts at 1, 2, and 4 weeks after fat transplantation showed colocalization of NLRP3 and MAC-2 in the indicated groups. Arrows denote NLRP3-positive macrophages. Scale bar: 50 μm. Graph shows the ratio of NLRP3-positive macrophage in the indicated groups. (H, I) WB analysis and quantification of NLRP3, GSDMD, IL-18, IL-1β, and caspase-1 protein levels were conducted in each group of fat grafts after 2 weeks of fat grafting. GAPDH was utilized as a loading control. (J-P) Double immunofluorescence staining images of fat grafts at 1, 2, and 4 weeks after fat transplantation showed colocalization of cleaved-caspase-1 and MAC-2 in the indicated groups. Arrows denote cleaved-caspase-1-positive macrophages. Scale bar: 50 μm. Graph shows the ratio of cleaved-caspase-1-positive macrophage in the indicated groups. (Q-W) Double immunofluorescence staining images of fat grafts at 1, 2, and 4 weeks after fat transplantation showed colocalization of IL-18 and MAC-2 in the indicated groups. Arrows denoted IL-18–positive macrophages. Scale bar: 50 μm. Graph shows the ratio of IL-18–positive macrophage in the indicated groups. The data are expressed as the mean ± SD ( n = 3). * P < .05, ** P < .01, *** P < .001. DSF, disulfiram; GSDMD, gasdermin D; IL, interleukin; SD, standard deviation; WB, Western blot.

Article Snippet: In brief, sections were incubated at 4°C overnight with mouse anti-mouse Galectin-3 antibody MAC-2 (cat. 60207-1-Ig, Proteintech, Wuhan, China, 1:1600); rabbit anti-mouse CD206 (cat. DF4149, Affinity, Jiangsu, China, 1:200); rabbit anti-mouse NLRP3 (cat. DF7438, Affinity, 1:100); rabbit anti-mouse IL-18 (cat. DF6252, Affinity, 1:400); and rabbit anti-mouse cleaved-caspase-1 (cat. AF4022, Affinity, 1:200) primary antibodies, and then with Alexa Fluor 448-conjugated goat anti-mouse (cat. A23210, Abbkine, Wuhan, China, 1:200) and Alexa Fluor 594-conjugated goat anti-rabbit (cat. A23420, Abbkine, Wuhan, China, 1:200) secondary antibodies.

Techniques: Double Immunofluorescence Staining, Transplantation Assay, Standard Deviation, Western Blot

DSF regulates macrophage polarization in fat grafts. (A-D) RT-qPCR quantified mRNA expression of M1 macrophage polarization-related cytokines (iNOS, TNF-α) and M2 macrophage polarization-related cytokines (Arg-1, IL-10) in fat grafts. (E-M) Double immunofluorescence staining images of fat grafts at 1, 2, 4, and 12 weeks after fat transplantation showed colocalization of M2 macrophage marker CD206 and MAC-2 in the indicated groups. Arrows denote M2 macrophages. Scale bar: 50 μm. Graph shows the ratio of M2 macrophage in the indicated groups. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01, *** P < .001. DSF, disulfiram; iNOS, inducible nitric oxide synthase; RT-qPCR, quantitative reverse transcription polymerase chain reaction; SD, standard deviation; TNF, tumor necrosis factor.

Journal: Aesthetic Surgery Journal

Article Title: Disulfiram Improves Fat Graft Retention by Modulating Macrophage Polarization With Inhibition of NLRP3 Inflammasome-Mediated Pyroptosis

doi: 10.1093/asj/sjae075

Figure Lengend Snippet: DSF regulates macrophage polarization in fat grafts. (A-D) RT-qPCR quantified mRNA expression of M1 macrophage polarization-related cytokines (iNOS, TNF-α) and M2 macrophage polarization-related cytokines (Arg-1, IL-10) in fat grafts. (E-M) Double immunofluorescence staining images of fat grafts at 1, 2, 4, and 12 weeks after fat transplantation showed colocalization of M2 macrophage marker CD206 and MAC-2 in the indicated groups. Arrows denote M2 macrophages. Scale bar: 50 μm. Graph shows the ratio of M2 macrophage in the indicated groups. The data are expressed as the mean ± SD ( n = 3). NS, not significant ( P > .05), * P < .05, ** P < .01, *** P < .001. DSF, disulfiram; iNOS, inducible nitric oxide synthase; RT-qPCR, quantitative reverse transcription polymerase chain reaction; SD, standard deviation; TNF, tumor necrosis factor.

Article Snippet: In brief, sections were incubated at 4°C overnight with mouse anti-mouse Galectin-3 antibody MAC-2 (cat. 60207-1-Ig, Proteintech, Wuhan, China, 1:1600); rabbit anti-mouse CD206 (cat. DF4149, Affinity, Jiangsu, China, 1:200); rabbit anti-mouse NLRP3 (cat. DF7438, Affinity, 1:100); rabbit anti-mouse IL-18 (cat. DF6252, Affinity, 1:400); and rabbit anti-mouse cleaved-caspase-1 (cat. AF4022, Affinity, 1:200) primary antibodies, and then with Alexa Fluor 448-conjugated goat anti-mouse (cat. A23210, Abbkine, Wuhan, China, 1:200) and Alexa Fluor 594-conjugated goat anti-rabbit (cat. A23420, Abbkine, Wuhan, China, 1:200) secondary antibodies.

Techniques: Quantitative RT-PCR, Expressing, Double Immunofluorescence Staining, Transplantation Assay, Marker, Reverse Transcription Polymerase Chain Reaction, Standard Deviation